Paul J. Marangos's Brain Receptor Methodologies. Part A: General Methods and PDF

By Paul J. Marangos

ISBN-10: 012470350X

ISBN-13: 9780124703506

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Extra info for Brain Receptor Methodologies. Part A: General Methods and Concepts. Amines and Acetylcholine

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J. (1979). Life Sei. 24, 1739-1746. Hunston, D. L. (1975). Anal. Biochem. 63, 9 9 - 1 0 9 . Klotz, I. M. (1982). Science 217, 1247-1249. Kosterlitz, H. W . , Paterson, S. , and Robson, L. E. (1981). Br. J. Pharmacol. 73, 9 3 9 - 9 4 9 . Martin, W. , Eades, C. B . , Thompson, J. Α . , Huppier, R. , and Gilbert, P. E. (1976). J. Pharmacol. Exp. Ther. 197, 5 1 7 - 5 2 2 . Munson, P. J. (1981), " U s e r ' s Guide to LIGAND. A Program System for Fitting Multiple Ligand, Multiple Binding Site D a t a .

Human antrum fixed in benzoquinone vapor, three in serial sections stained for neuronspecific enolase (a) and for gastrin (b). Long arrows point to endocrine cells containing both peptide and enzyme. Other cells are clearly stained only for neuron-specific enolase, indicating the près- 51 4. The Distribution of Peripheral Regulatory Peptides A.

To determine if these ligands are binding to the same or to independent classes of receptors, competition studies using the corresponding unlabeled ligands should be performed. Complete cross-displacement should be observed if only a single receptor binding site is involved. Failure of one of the unlabeled ligands to compete or reduction of the slope of the competition curve suggests that two or more independent sites may be involved. Saturation of the same receptor with different labeled ligands should result in the same measured binding capacity.

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Brain Receptor Methodologies. Part A: General Methods and Concepts. Amines and Acetylcholine by Paul J. Marangos


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